Fig. 3

Expression of DRAM2 affected the migration of non-small cell lung cancer (NSCLC) cells. a. Transwell migration assays were performed to analyze cell migration in the context of DRAM2 overexpression and downregulation in SK and A549 cells, respectively. Cells that migrated to the lower chamber were stained with hematoxylin and counted. DRAM2 overexpression enhanced cell migration and DRAM2 knockdown inhibited cell migration. p-values for SK were: DRAM2 vs PCMV6, p < 0.0001; SiDRAM2 vs negative control siRNA, p = 0.0002. p-values for A549 were: DRAM2 vs PCMV6, p = 0.0001; SiDRAM2 vs NC, p = 0.0019. *p < 0.05, **p < 0.01, ***p < 0.001. b. Effects of DRAM2 levels on the expression of proteins associated with cell migration in transfected SK and A549 cells. The gray level of the proteins were detected, the corresponding p value were provided at the top of the graphs and those changed proteins were marked.*p < 0.05, **p < 0.01, ***p < 0.001