Fig. 4

Endogenous expression of FOXK1 and effect of FOXK1 on the biological behaviors of glioma cells. a Protein levels of FOXK1 in NBTs, LGGTs and HGGTs. Representative protein expressions and corresponding IDVs of FOXK1 in NBTs (n = 15), low-grade glioma (n = 15), and high-grade glioma (n = 15) are shown; data are presented as mean ± SD.^**P < 0.01 vs. NBTs group, ^##P < 0.01 vs. LGGTs group. b Protein levels of FOXK1 in NHA, U87 and U251 cells. Data are presented as mean ± SD (n = 3, each group). ^**P < 0.01 vs. NHA group. c Gene expression difference curve according to TCGA. d Survival analysis curve according to TCGA. e Effects of ZRANB2 on FOXK1 expression; data are presented as mean ± SD (n = 3, each group). ^*P < 0.05 vs. ZRANB2(−)-NC group, ^**P < 0.01 vs. ZRANB2(−)-NC group, ^##P < 0.01 vs. ZRANB2(+)-NC group. f Effects of SNHG20 on FOXK1 expression; data are presented as mean ± SD (n = 3, each group). ^*P < 0.05 vs. SNHG20(−)-NC group, ^##P < 0.01 vs. SNHG20(+)-NC group. g FOXK1 expression regulated by ZRANB2 and SNHG20; data are presented as mean ± SD (n = 3, each group). ^*P < 0.05 vs. ZRANB2(−) + SNHG20(+) group, ^#P < 0.05 vs. ZRANB2(+) + SNHG20(−) group, ^##P < 0.01 vs. ZRANB2(+) + SNHG20(−) group. h Protein levels of MMP1, MMP9 and VE-Cadherin regulated by FOXK1. Data are presented as mean ± SD (n = 3, each group). ^*P < 0.05 vs. FOXK1(−)-NC group, ^**P < 0.01 vs. FOXK1(−)-NC group, ^#P < 0.05 vs. FOXK1(+)-NC group, ^##P < 0.01 vs. FOXK1(+)-NC group. i CCK-8 assay. Data are presented as mean ± SD (n = 3, each group). ^*P < 0.05 vs. FOXK1(−)-NC group, ^**P < 0.01 vs. FOXK1(−)-NC group, ^#P < 0.05 vs. FOXK1(+)-NC group, ^##P < 0.01 vs. FOXK1(+)-NC group. j Transwell assay. Representative images and corresponding statistical plots are shown. Data are presented as mean ± SD (n = 3, each group). ^**P < 0.01 vs. FOXK1(−)-NC group, ^#P < 0.05 vs. FOXK1(+)-NC group, ^##P < 0.01 vs. FOXK1(+)-NC group. Scale bars indicate 50 μm. k Three-dimensional culture. Data are presented as mean ± SD (n = 3, each group). ^*P < 0.05 vs. FOXK1(−)-NC group, ^#P < 0.05 vs. FOXK1 (+)-NC group. Scale bars indicate 50 μm