Fig. 5

SNHG420 strengthened the malignant behaviors of glioma cells by degrading FOXK1 mRNA through SMD pathway. a The predicted SNHG20 binding site in FOXK1 mRNA 3’UTR and results of dual-luciferase reporter assays. Data are presented as mean ± SD (n = 3, each group). ^*P < 0.05 vs. FOXK1–3’UTR-Wt + SNHG20(+)-NC group. b RNA-IP results. Data are presented as mean ± SD (n = 3, each group). ^**P < 0.01 vs. anti-normal IgG group. c RNA-IP results. Data are presented as mean ± SD (n = 3, each group). ^**P < 0.01 vs. anti-normal IgG group. d Stability of FOXK1 mRNA by SNHG20; data are presented as mean ± SD (n = 3, each group). ^**P < 0.01 vs. SNHG20(−)-NC group, ^##P < 0.01 vs. SNHG20(+)-NC group. e Stability of FOXK1 mRNA by STAU1; data are presented as mean ± SD (n = 3, each group). ^**P < 0.01 vs. SNHG20(−)-NC group. f Stability of FOXK1 mRNA by UPF1; data are presented as mean ± SD (n = 3, each group). ^**P < 0.01 vs. SNHG20(−)-NC group. g Effect of STAU1 on FOXK1 expression; data are presented as mean ± SD (n = 3, each group). ^*P < 0.05 vs. STAU1(−)-NC group, ^**P < 0.01 vs. STAU1(−)-NC group. h Effect of UPF1 on FOXK1 expression; data are presented as mean ± SD (n = 3, each group). ^*P < 0.05 vs. UPF1(−)-NC group. i Protein levels of MMP1, MMP9 and VE-Cadherin. Data are presented as mean ± SD (n = 3, each group). ^*P < 0.05 vs. SNHG20(−) + FOXK1(−) group, ^**P < 0.01 vs. SNHG20(−) + FOXK1(−) group, ^#P < 0.05 vs. SNHG20(+) + FOXK1(+) group, ^##P < 0.01 vs. SNHG20(+) + FOXK1(+) group. (J) CCK-8. Data are presented as mean ± SD (n = 3, each group). ^**P < 0.01 vs. FOXK1(−)-NC group, ^#P < 0.05 vs. FOXK1(+)-NC group, ^##P < 0.01 vs. FOXK1(+)-NC group. k Transwell. Data are presented as mean ± SD (n = 3, each group). ^*P < 0.05 vs. SNHG20(−) + FOXK1(−) group, ^**P < 0.01 vs. SNHG20(−) + FOXK1(−) group, ^#P < 0.05 vs. SNHG20(+) + FOXK1(+) group, ^##P < 0.01 vs. SNHG20(+) + FOXK1(+) group. Scale bars indicate 50 μm. l Three-dimensional culture. Data are presented as mean ± SD (n = 3, each group). ^*P < 0.05 vs. SNHG20(−) + FOXK1(−) group, ^**P < 0.01 vs. SNHG20(−) + FOXK1(−) group, ^##P < 0.01 vs. SNHG20(+) + FOXK1(+) group. Scale bars indicate 50 μm