Fig. 4

EVI1 regulated CSC properties in NPC cells. (a) PKH26 + − and ALDH1 + −labeled cells were significantly decreased in sh-EVI1–5-8F and sh-EVI1-CNE-2 cells compared with those in sh-ctrl cells. However, when EVI1 was overexpressed in 6-10B cells, the PKH26 + − and ALDH1 + −labeled cells were significantly increased. (b) The sphere formation ability was impaired in sh-EVI1–5-8F and sh-EVI1-CNE-2 cells but was enhanced in LV-EVI1–6-10B cells. (c) The expression levels of SOX2, Nanog and c-myc were decreased in sh-EVI1–5-8F and sh-EVI1-CNE-2 cells but were enhanced in LV-EVI1–6-10B cells