Fig. 2

MiR-9 promotes angiogenesis, adhesion, migration and invasion of HUVECs. a MiR-9 expression levels in the serum of normal individuals (N, n = 8) and glioma patients (G, n = 22) were analyzed by using the data from GSE93850. Data are shown as the mean ± s.d. (**P < 0.01). b CD31 IHC staining was performed in normal (N) and glioma (G) tissues we collected. Scale bars represent 100 μm (upper) and 200 μm (lower). c Amount of capillary-like tubes generated by the HUVEC miR-9 mimic/NC cells was counted under a microscope 48 h post transfection. Scale bars represent 200 μm. Data are shown as the mean ± s.d. (**P < 0.01; n = 3 independent experiments). d Number and length of the novel sprouts derived from HUVEC miR-9 mimic/NC and HUVEC miR-9 inhibitor/NC cells were examined under a microscope. Scale bars represent 100 μm. Data are represented as the mean ± s.d. (**P < 0.01 and ***P < 0.001; n = 3 independent experiments). e Cell adhesive force assay was utilized to evaluate the adhesion ability of HUVEC miR-9 mimic/NC cells at 0.5, 1, 2 and 4 h. Data are shown as the mean ± s.d. (*P < 0.05, **P < 0.01 and ***P < 0.001; n = 3 independent experiments). Scale bars represent 500 μm. f Migration and invasion of the HUVEC miR-9 mimic/NC cells was determined through non-coated (upper) and Matrigel-coated (lower) transwell assays. Data are shown as the mean ± s.d. (**P < 0.01; n = 3 independent experiments). Scale bars represent 100 μm