Fig. 4

Depletion of LPCAT1 inhibited the tumorigenesis and brain metastasis in vivo. a, b HCC827 and PC-9 cells stably transfected with NC and shLPCAT1 were injected subcutaneously into nude mice. Four weeks after the injection, mice were photographed and killed. Tumor growth curves were plotted. c Gene set enrichment analysis (GSEA) showed that amplification of LPCAT1 was correlated with the geneset of “GNF2_PCNA” in LUAD patients. d, e Representative IHC staining and quantitative analysis of PCNA, CD34 and MMP9 in xenograft tumors. f, g Representative bioluminescent/photographic images (f) and HE images (100×, 200×, g) in brain metastatic mice. The data are expressed as the mean ± SD, *P < 0.05, **P < 0.01, ^# P < 0.001, as determined by the t-test