Fig. 1

Anlotinib inhibited proliferation and induced apoptosis of human lung cancer cells. a, Dose-response curves of Calu-1 and A549 cells to anlotinib treatment. Cells were cultured by anlotinib at various concentrations for 24, 48 and 72 h and cell viability was detected by CCK-8 assay. b, Representative images of clone formation assay. c, Effect of anlotinib on the nuclear morphology of lung cancer cells after anlotinib (24 h) treatment was detected by Hoechst-33,258 staining. Apoptotic bodies are indicated by white arrows. d, The ratio of apoptotic cells was measured in Calu-1 and A549 cells after anlotinib treatment for 24 h. Apoptosis was detected by Annexin V-FITC and propidium iodide (PI) staining. The columns represent means ± SD of three independent experiments. *P < 0.05, **P < 0.01. Each experiment was performed in triplicate. Scale bar: 100 μm