Fig. 1

Hypoxic EOC cells induced macrophages into a TAM-like phenotype. a Changes of HIF-1α expression in EOC cells in exposure to hypoxia for 4 or 8 h. b After cocultured with normoxic or hypoxic EOC cells (exposure to hypoxia for 4 or 8 h) for 24 h, migration ability of macrophages was detected (× 200 magnification). c HIF-1α expression in EOC cells and macrophages after exposure to hypoxia together for 4 or 8 h. d After exposure to hypoxia for 4 or 8 h, macrophages were cocultured with normoxic or hypoxic EOC cells for an additional 24 h, then, migration ability of macrophages was detected. e Flow cytometry expression of CD163 in macrophages alone (control, left panels) or cocultured with EOC cells (coculture, right panels) for 48 h. f The related fold change of CD206,Arg-1 and MCP-1 mRNA in macrophages alone or cocultured with normoxic or hypoxic SKOV3 cells. g IL-10 and IL-12 expression levels in macrophages were evaluated by ELISA. *P < 0.05, **P < 0.01