Fig. 4

Chemical inhibition of SHP1 and SHP2 promotes differentiation in HL-60 cells. a) Silencing of both SHP1 and SHP2 in HL-60 cells. b) CD11b expression in SHP1- and SHP2-silenced HL-60 cells after treatment with PMA 20 nM during the indicated times. c) CD11b expression in HL-60 cells after 48 h treatment with SSG and NSC. d) CD11b expression in HL-60 cells after 48 h treatment with PMA, NSC and their combination. e) CD11b expression in HL-60 cells after 48 h treatment with PRS, NSC and their combination. f) Representative pictures of May-Grünwald-Giemsa stained cytospins of HL-60 cells after 48 h treatment with PRS, NSC and their combination. Scale bar: 10 μm