Fig. 7

TRIM29 mediates EMT and invasion via activating the Wnt/β-catenin signaling pathway. a Modified RKO cells were treated with XAV939 (15 μM) for 24 h, and the indicated protein levels were then assayed by Western blotting. laminB1, β-actin, and α-Tubulin were used as internal controls. b The β-catenin protein expression and localization in RKO-Vector and RKO-TRIM29 cells with XAV939 (15 μM) treatment was assessed by immunofluorescence assay. c The Transwell invasion assay showed different cell invasive abilities in RKO-Vector and RKO-TRIM29 cells with XAV939 (15 μM) for 24 h. The data are presented as the mean ± SEM. from three independent experiments (**P < 0.01, ***P < 0.001)