Fig. 1
The potential involvement of the LINC00968/HEY1/WNT2 axis mediated Wnt2/β-catenin signaling pathway in breast cancer. a selection thermogram of GEO dataset GSE26910. The horizontal coordinate represents the samples, and the vertical coordinate represents the genes. The upper right histogram is the color gradation. Each rectangle corresponds to one gene expression in one sample. Each column signifies gene expression of each sample; dendrogram on the left side represents cluster analysis of different genes from different samples; uppermost crossband represents sample type and upper right pane signifies color reference, while blue is the normal control sample and red is tumor sample. b the expression pattern of LINC00968 in cancer tissues and normal tissues of various cancers according to TCGA database. c the expression of LINC00968 in breast cancer tissues and adjacent normal tissues. d the survival analysis of LINC00968 expression and survival rate of patients. e the subcellular localization of LINC00968 in breast cancer cells as detected by FISH assay. f the binding status of LINC00968 to HEY1 as confirmed by RIP assay. g the binding sites of HEY1 to the WNT2 promoter as predicted by online Jaspar website (http://jaspar.genereg.net/). h the binding status of HEY1 to the WNT2 promoter as verified by ChIP assay. i the interaction between LINC00968 and WNT2 as verified by Dual luciferase reporter gene assay. *p < 0.05 vs. the NC group. j the mRNA expression of INC00968 and WNT2 as determined by RT-qPCR. k the protein levels of INC00968 and WNT2 as evaluated by Western blot analysis. l the expression of WNT2 in cell culture medium as assayed by ELISA. *p < 0.05 vs. the control group; # p < 0.05 vs. the blank and NC groups; & p < 0.05 vs. the LINC00968 vector group. m the mRNA expression of LINC00968, WNT2, and β-catenin in breast cancer tissues and normal adjacent tissues as measured by RT-qPCR. n the protein level of WNT2 and the phosphorylation levels of β-catenin and GSK3β. o the level of LINC00968 determined by Northern blot analysis. *p < 0.05 vs. the adjacent normal tissues