Fig. 6

TFE3 fusion proteins are increased in the cytoplasm as a result of inhibition of the ubiquitination degradation or activation of Gsk3β. (a) UOK120 cells were treated with AKT inhibitor (MK2206, 5 μM) or vehicle control (Control) for 24 h. Expression levels of TFE3 (red) was examined by immunofluorescence microscopy. Nucleus were stained with DAPI (blue). Scale bar, 50 μm. (b) HK-2 cells viability were detected by CCK-8 after treatment with MG-132 or vehicle control (Con) for 24 h. (c) Expression levels of TFE3 were examined by immunoblot analysis. HK-2 cells were treated with PP242 (2 μM) or MG-132 (500 nM) for 24 h