Fig. 2

miR-200b inhibits fascin-1 (FSCN1) expression, migration, invasion. a Luciferase assays were performed after T24, RT4 and 293 T cells were co-transfected for 24 h with miR-negative control (miR-NC) or miR-200b and a plasmid containing wild-type or mutant-type FSCN1 3′ untranslated region (UTR) upstream the luciferase gene. The firefly luciferase activity of each sample was normalized by Renilla luciferase activity. Data were analyzed by T-test. b The mRNA level of FSCN1 in T24 and RT4 cells was measured by quantitative PCR (qPCR) after miR-200b was silenced or overexpressed. Data were analyzed by T-test. c The protein level of E-cadherin, N-cadherin, vimentin and FSCN1 in T24 and RT4 cells was measured by western blot after miR-200b was silenced or overexpressed. Data were analyzed by T-test. d The protein level of FSCN1 in T24 cells was measured by western blot after the cells were co-transfected with ant miR-NC or ant miR-200b and siNC or siFSCN1. Data were analyzed by T-test. e and f The migration (e) and invasion (f) abilities of T24 and RT4 cells were detected in transwell assays (without or with Matrigel) after miR-200b was silenced or overexpressed. g and h The cell migration (g) and invasion (h) abilities of T24 and RT4 were detected in transwell assays (without or with Matrigel) after co-transfection with miR-NC or miR-200b and siNC or siFSCN1. Data were analyzed by T-test. All images were taken at 100× magnification. Data are presented as the mean ± standard deviation (SD). *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant