Fig. 6
From: MicroRNA-137 reduces stemness features of pancreatic cancer cells by targeting KLF12
KLF12 transcriptionally regulates DVL2–β-catenin signaling in pancreatic cancer cells. a TCGA analysis showing that the expression correlation of KLF12 and DVL1,DVL2,DVL3 in the TCGA pancreatic cancer data set. b RT–qPCR analysis of the expression of DVL1,DVL2,DVL3 in the indicated cells c Bioinformatics analysis showing that the DVL2 promoter contains two putative KLF12 DNA-binding motifs . d Chromatin Immunoprecipitation assays indicated that KLF12 may bind to the promoter regions of DVL2.e RT–qPCR analysis of the expression of the established downstream targets for the Wnt/β-catenin pathway in the indicated cells. e The expression of Wnt-activated /suppressed gene and nuclear β-catenin in the indicated cells, as determined by western blotting