Fig. 5

CRIP1 stimulates the ubiquitination and degradation of Fas. a. Real-time PCR shows the effects of CRIP1 on the expression of Fas at transcriptional level in both SW620 and HCT116 cells. b. Co-Ip shows the interaction between CRIP1 and Fas in both SW620 and HCT116 cells. c. The immunofluorescence staining demonstrated the co-localization of CRIP1 and Fas in both SW620 and HCT116 cells. d. Left panel: western blot assay shows that MG-132 recovered the inhibitory role of CRIP1 on Fas Protein. Right panel: the effects of MG-132 alone on the expression of Fas protein. e. CRIP1 stimulates the ubiquitination of Fas, while both siCRIP1 and MG-132 could recover the ubiquitin-mediated degradation of Fas