Fig. 1

DNT cells infiltrate lung cancer and are cytotoxic to lung cancer cells. Flow cytometric analysis of T cells from different lung tissue compartments, cancer (CA), adjacent (ADJ), or grossly normal lung tissue (NOR), resected from lung cancer patients (n = 10). a Representative contour plots of lung tissue-derived T cell subsets by gating on CD3⁺ cells. b Frequency of lung tissue derived T cell subsets, each point represents data from an individual patient. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA. Representative flow cytometry plot and cumulative frequency of DNT (c), CD4 (d) and CD8 (e) T cell subsets expressing CD45RA and CD27, presented as mean ± SEM of 9 evaluable patients. *p < 0.05, **p < 0.01, ***p < 0.001 by two-tailed unpaired t-test relative to cancer (CA) tissue. f Lung cancer patient-derived or healthy donor-derived DNT cells were co-cultured with indicated lung cancer cell lines at various DNT cell to tumor ratios. % specific killing of target cells is shown. The results represent two independent experiments each with triplicate cultures