Fig. 3

Downregulating ANXA2 expression inhibited GBM cells proliferation, cell cycle and apoptosis in vitro and vivo. a Colony formation assays in U87 and Primary glioblastoma cells (GP1) transfected with scramble, ANXA2 siRNA 1 and siRNA 2. Scale bar> 500 μm. b U87 and GP1 in EDU transfected with scramble, ANXA2 siRNA 1 and siRNA 2 after 48 h. Representative merged images were shown (original magnification, 200×). c The cell cycle was detected in U87 and GP1 transfected with scramble, ANXA2 siRNA 1 and siRNA 2 after 48 h. d The apoptotic cells were measured with flow cytometry in U87 and GP1 transfected with scramble, ANXA2 siRNA 1 and siRNA 2 after 48 h. e The protein related with cell proliferation, cell cycle and apoptosis was measured with immunoblotting in U87 and GP1 cells transfected with scramble, ANXA2 siRNA 1 and siRNA 2 after 48 h. All experiments above were performed 3 times, and average scores are indicated with error bars on the histogram.*P < 0.05, **P < 0.01. f U87 cells transfected with a lentivirus with sh-ANXA2 or sh-NC and a lentivirus containing luciferase were implanted in the brain of 10 nude mice, respectively. The tumor formation was assessed by bioluminescence imaging. The bioluminescent images were measured at days 1, 11 and 21 after implantation. g Overall survival was determined by Kaplan-Meier survival curves between sh-ANXA2 or sh-NC group, and a log-rank test was used to assess the statistical differences. h Two representative immunohistochemical image of tumors from groups of nude mice implanted with U87 cells, transfected with a lentivirus with sh-ANXA2 or sh-NC, were shown to compare the volume size of tumors