Fig. 1

MCUR1 promoted EMT of HCC cells. a Immunofluorescent image of epithelial markers (ZO-1 and E-cadherin) and mesenchymal markers (N-cadherin and Vimentin) in HCC cells with different MCUR1 expression levels. Cells were transfected with siRNA or expression vector for 48 h. Scramble: vector encoding control shRNA; shMCUR1 vectors encoding short hairpin RNA (shRNA) against MCUR1. EV: Empty Vector; MCUR1: expression vectors encoding MCUR1; Snail, Snail expression vector; siSnail, siRNA against Snail. b Western blot analysis of ZO-1, E-cadherin, N-cadherin, Vimentin, Snail and Slug in HCC cells with different MCUR1 expression levels