Fig. 5
Activation of ROS/Nrf2/Notch pathways is essential for MCUR1-induced EMT and HCC metastasis. a, c Western blot analysis for expression levels of Snail, ZO-1, E-cadherin, N-cadherin and Vimentin in stably transfected HCC cells with treatments as indicated. b, d Transwell assay for migration and invasion ability and wound healing assays for migration rate in stably transfected HCC cells with treatment as indicated. H2O2 (25 μM, for 1 h), OPZ (20 μM, for 1 h), MitoTEMPO (5 μM for 1 h), DAPT (2 μM, for 6 h); cells were transfected with siRNA or expression vector for 48 h. NICD1, NICD1 expression vector; siNrf2, siRNA against Nrf2. Migration rate% = (Migrated distance at indicated time - initial distance) / (initial distance) × 100%. Data shown are the mean ± SD from three independent experiments. * P < 0.05; ** P < 0.01