Fig. 2

LncSBF2-AS1 overexpression confers TMZ resistance. a LncSBF2-AS1 transfected Pri GBM and N3S cells and elevated SBF2-AS1 expression. The data represents the mean ± SEM from three independent experiments. ***P < 0.001. b Colony formation ability of SBF2-AS1 overexpressing TMZ-sensitive cells or vector control cells in the absence or presence of TMZ. Data represents mean of three independent experiments ± SEM. ***P < 0.001. c Flow cytometry was used to measure the apoptosis of TMZ-sensitive SBF2-AS1 overexpression cells or vector control cells after exposure to 200 μM TMZ for 48 h. Data represents means of three independent experiments ± SEM. ***P < 0.001. d Western blot analysis of cleaved caspase-3 expression of TMZ-sensitive cells overexpressing SBF2-AS1 or vector control cells in the absence or presence of TMZ. Tubulin was used as the loading control. e TUNEL analysis of TMZ-sensitive cells overexpressing SBF2-AS1 or vector control cells in the absence or presence of TMZ for 48 h. Data represents means of three independent experiments ± SEM. ***P < 0.001. Scale bar, 50 μm. f Representative images of Pri GBM cells overexpressing SBF2-AS1 or vector control cells-derived subcutaneous tumors on 42nd day in the absence or presence of TMZ. g Growth curve of tumor xenografts originated from SBF2-AS1-overexpressing Pri GBM cells or vector control cells in the absence or presence of TMZ. Data represents means of three independent experiments ± SEM.*P < 0.05, **P < 0.01, ***P < 0.001. h Weight of tumor xenografts originated from SBF2-AS1-overexpressing Pri GBM cells or vector control cells in the absence or presence of TMZ. Data represents means of three independent experiments ± SEM. **P < 0.01. i IHC analysis of cleaved caspase-3 in SBF2-AS1-overexpressing Pri GBM cells or vector control cells-derived subcutaneous tumors in the absence or presence of TMZ. Scale bar, 50 μm