Fig. 4

Endogenous ZEB1 regulated SBF2-AS1 at transcriptional level. a Each individual ZEB1 and SBF2-AS1 expression was analyzed by linear regression analysis. b ZEB1 expression in recurrent ZEB1-knockdown Rec GBM cells and ZEB1-overexpressed Pri GBM cells were analyzed by Western blot assay. β-actin was used as the loading control. c SBF2-AS1 expression in Rec GBM cells and Pri GBM cells were analyzed by qRT-PCR. Data represents means of three independent experiments ± SEM. **P < 0.01. d Schematic diagram showing the human SBF2-AS1 promoter region. e CHIP assay was performed to explore the relative enrichment of ZEB1 on promoter region of lncSBF2-AS1. This figure shows the results. f Western blot analysis of cleaved caspase-3 expression of ZEB1-overexpressed Pri GBM cells or ZEB1-knockdown GBM cells in the absence or presence of TMZ. Tubulin was used as the loading control. g TUNEL analysis of ZEB1-overexpressed Pri GBM cells or ZEB1-knockdown GBM cells in the absence or presence of TMZ for 48 h. Data represents means of three independent experiments ± SEM. **P < 0.01. Scale bar, 50 μm. h Flow cytometry was used to measure the apoptosis of ZEB1-overexpressed Pri GBM cells or ZEB1-knockdown GBM cells after exposure to 200 μM TMZ for 48 h. Data represents means of three independent experiments ± SEM. ***P < 0.001