Fig. 8

Exosomal transfer of lncSBF2-AS1 enhances chemoresistance to TMZ in vivo. a Representative bioluminescence images of intracranial xenografts derived from Pri GBM, which were pre-incubated with PBS or 50 μg/ml Rec GBM-exo or Rec GBM-exo (shSBF2-AS1) for 2 days. b Survival curves of nude mice derived from Pri GBM cells indicated above in the absence or presence of TMZ. c Bioluminescence was measured in tumors from six groups. Data represents means of three independent experiments ± SEM. *P < 0.05, **P < 0.01. d TUNEL staining of intracranial xenografts derived from Pri GBM cells indicated above in response to TMZ. Scale bar, 50 μm (e) The expression of lncSBF2-AS1, miR-151a-3p, XRCC4, γ-H2AX, and cleaved caspase-3 were analyzed by FISH or IHC analysis on intracranial xenografts derived from Pri GBM cells indicated above in response to TMZ. Scale bar, 50 μm (left, FISH) and 50 μm (right, IHC)