Fig. 9

Expression of serum exosomal lncSBF2-AS1 is associated with TMZ resistance in GBM patients. a Representative transmission electron microscopy (TEM) of serum-derived exosomes from recurrent GBM patients and primary GBM patients. Scale bar, 100 nm. b Western blot assay for exosomal markers CD63, CD81 and GM130 of serum-derived exosomes from recurrent GBM patients and primary GBM patients. c qRT-PCR analysis of lncSBF2-AS1 expression in serum-derived exosomes from recurrent GBM patients and primary GBM patients. Data represents means of three independent experiments ± SEM. d Survival curves of 20 GBM patients who received TMZ treatment, with low (n = 10) and high (n = 10) serum exosomal lncSBF2-AS1 levels. The number of primary patients (P) and recurrent patients (R) per survival group were indicated. e Immunohistochemical staining of cleaved caspase-3 in GBM samples with high exosomal lncSBF2-AS1 levels or low exosomal lncSBF2-AS1 levels. Scale bar, 50 μm