Fig. 3

TRIM47 promotes proliferation of colorectal cancer cells in vitro and in vivo. a and b Cell proliferation assay was performed in HCT116 and HT29 cells transfected with control siRNA and TRIM47 siRNAs (n = 3, non-parametric Mann-Whitney test, P < 0.05). c Cell proliferation assay was performed in SW480 cells transfected with vector plasmids and TRIM47 overexpression plasmids (n = 3, non-parametric Mann-Whitney test, P < 0.05). d and e Colony forming assay was performed in HCT116, HT29 and SW480 cells (n = 3, non-parametric Mann-Whitney test, P < 0.05). f Proliferation related proteins c-Myc and Cyclin D1 were detected in HCT116, HT29 and SW480 cells by western blot (n = 3). g Representative images of tumors in nude mice bearing colorectal cancer cells treated with PBS, control shRNA adenovirus and TRIM47 shRNA adenovirus (n = 8). h Tumor volume was measured after different treatments in nude mice (n = 8, non-parametric Mann-Whitney test, P < 0.05). i Tumor weight was measured in nude mice after different treatments (n = 8, non-parametric Mann-Whitney test, P < 0.05). j Representative images of Ki67 IHC staining of the three groups mice xenografts (n = 8, non-parametric Mann-Whitney test, P < 0.05). k Proliferation related proteins c-Myc and Cyclin D1 were detected in mice xenografts by western blot (n = 3)