Fig. 6

RASSF1A depletion induces YAP-dependent cytokinesis defect. HBEC-3 cells were transfected with si-RASSF1A, siYAP and/or si-Neg. Cells were stained with anti-RASSF1A, anti-tubulin and/or anti-AuroraB antibodies and DAPI. Persistent midbody was quantified (a) as the number of cells failing to divide following cytokinesis defect by scoring > 100 cells, imaged at 2 min intervals when rounded up (b). c Percentage of HBEC-3 cells multinucleated and/or with persistent midbody evaluated following RASSF1A and YAP silencing and immunostaining of the alpha-tubulin with DAPI for the nucleus. a, c Scale bar represents 50 μm. Error bars indicate the SEM (n ≥ 3). *P < 0.05, **P < 0.01 and ***P < 0.001, using an ANOVA test followed by Dunnett’s test. b Correlation between RASSF1A presence and events was test using a Chi2 test