Fig. 4
From: Sox11 promotes head and neck cancer progression via the regulation of SDCCAG8
Knockdown of SOX11 in head and neck cancer cells inhibits the expression of SDCCAG8 tumor antigen. (a) Quantitative proteomic analysis of UM1 or UMSCC5 cells transfected with siCTRL (UM1: TMT128; UMSCC5: TMT130) or siSox11 (UM1: TMT129; UMSCC5: TMT131). The TMT129:TMT128 ratios (y-axis) represent the relative levels of cellular proteins between UM1-siCTRL and UM1-siSox11 whereas the TMT131:TMT130 ratios represent the relative levels of cellular proteins between UMSCC5-siCTRL and UMSCC5-siSox11. (b) Western blot analysis of SOX11 and SDCCAG8 in UM1 or UMSCC5 cells transfected with siSox11 or siSdccag8. (c) Western blot analysis of SDCCAG8 in UM1, UM2, UMSCC5 and UMSCC6 cells (n = 3, ***, P < 0.001). (d) qPCR analysis of Sdccag8 gene expression in UM1, UM2, UMSCC5 and UMSCC6 cells (n = 3, ***, P < 0.001)