Fig. 5
From: Sox11 promotes head and neck cancer progression via the regulation of SDCCAG8
SOX11 binds to Sdccag8 gene promoter and induces Sdccag8 gene promoter activity. (a) ChIP-qPCR analysis of anti-SOX11- or IgG-immunoprecipitated DNA fragments from UM1 or UMSCC5 cells. (b) Luciferase reporter assays of UM2 or UMSCC6 cells transfected with empty promoter reporter vector, Sdccag8 gene promoter reporter (Sdccag8-Prom), Sdccag8-Prom and Sox11F, or Sdccag8-Prom and Sox11FΔTAD, respectively. Data are measured in triplicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (c) Western blot analysis of SOX11 and SDCCAG8 in UM1/UMSCC5 cells transfected with Sox11FΔTAD (mutant) and UM2/UMSCC6 cells transfected with Sox11F (WT)