Fig. 5

PHF14 was identified as a direct target of miR-590 in BC cells. a miR-590 and miR-NC were transfected into T24 cells. RT-qPCR was performed to quantify the RNA levels of several candidates. *P < 0.01. b Schematic diagram showing the predicted miR-590 binding sites within the 3’UTR of PHF14. The sequences of wild-type and mutant 3’UTR of PHF14 are also listed. Luciferase reporter gene assays were performed to measure the luciferase activity in T24 cells. *P < 0.01. c Biotinylated miR-590 or its mutant (miR-590-mut) was transfected into T24 cells. RT-qPCR was performed to quantify the RNA levels of the 3’UTR of PHF14 and GAPDH. Scatter plot showing the relative ratios of the input of IP. *P < 0.01. d The relative expressions of PHF14 in BC cells transfected with the Lv-LINC00162 vector/Lv-NC vector and shLINC00612 vector/shNC vector were measured via RT-qPCR. **P < 0.01. e, f RT-qPCR and western blots were performed to determine the relative expression of PHF14 in BC cell lines (5637, UMUC3, and T24) and human bladder epithelium immortalized cells (SV-HUC-1). ***P < 0.001. N = 3 independent experiments