Fig. 3

LINC01354 promoted proliferation and migration of CRC in vitro. a-b CCK8 and EdU assays were applied to examine cell proliferation ability in si-LINC01354#1/2 transfected LoVo cells and pcDNA3.1/LINC01354 transfected HCT116 cells. c The apoptosis rate in si-LINC01354#1/2 transfected LoVo cells and pcDNA3.1/LINC01354 transfected HCT116 cells was assessed by flow cytometry. d Transwell assays revealed the cell motility in LoVo cells transfected with si-LINC01354#1/2 and HCT116 cells transfected with pcDNA3.1/LINC01354. e-f Western blot and immunofluorescence were utilized to detect EMT associated proteins expression of E-cadherin and Vimentin in the two kinds of CRC cells. Results were exhibited as the mean ± SD on the basis of 3 independent experiments, ^**p < 0.01, ^***p < 0.001