Fig. 5

MiR-147a directly targets Slug to mediate EMT progression in NSCLC cells. a. Predicted binding sites for miR-147a in the slug mRNA sequence. b. Bioinformatics analysis of Slug expression in human lung adenocarcinoma and lung squamous cell carcinoma tumour tissues compared with normal tissues. c-d. Slug expression in SNH-treated A549 and NCI-H1299 cells as determined by qRT-PCR (c) and western blot analysis (d). e-f. Slug expression in SNH-treated A549 and NCI-H1299 cells after p-Linc00668 transfection as determined by qRT-PCR (e) and western blot analysis (f). g-h. Slug expression in sh-Linc00668–1-transfected NCI-H1299 cells as determined by qRT-PCR (g) and western blot analysis (h). i-j. Slug expression in miR-147a mimic-transfected A549 and NCI-H1299 cells as determined by qRT-PCR (i) and western blot analysis (j). The bars and error bars indicate the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001