Fig. 2

VPA significantly down-regulates the expression of ErbB family members EGFR, ErbB2, and ErbB3 and modulates the activation of downstream Akt and MAPK signalings. HPAF-II and MPanc96 cells untreated or treated with VPA (0.5 mmol/L) for 24 h or 48 h were harvested and subjected to western blot analyses with specific antibodies directed against EGFR, ErbB2, ErbB3, P-Akt, Akt, P-MAPK, MAPK, P-STAT3, STAT3, RAS or β-actin. The densitometry analyses of EGFR, ErbB2, and ErbB3 signals were shown underneath, and the arbitrary numbers indicate the intensities of each cell line relative to controls, defined as 1.0