Fig. 1

EMT and invasion potential are increased in cisplatin-resistant NSCLC cells. a Generation of cisplatin-resistant NSCLC cells. A549CisR and H157CisR cells were obtained by continuous treatment of A549P and H157 parental cells with increasing dose of cisplatin for 6 months. The IC50 of A549CisR and H157CisR and parental cells to cisplatin were analyzed in MTT assay. b Morphology alteration of cisplatin-resistant NSCLC cells. A549P/H157CisR H157P/H157CisR (1 × 10³) were cultured in normal media for 24 h and photos of cell morphology were obtained under microscope. c EMT alteration of cisplatin-resistant NSCLC cells. Total cell protein extracts were obtained from parental (A549P and H157P) and cisplatin-resistant cells (A549CisR and H157CisR) and Western blot analysis was performed using EMT-related antibodies indicated. d Increased Invasion potential in cisplatin-resistant NSCLC cells. Cells (A549P/A549CisR and H157P/H157CisR, 1 × 10⁴) were placed in upper chamber of transwell plates (8 μM pore) and invaded cells to lower chamber were counted under microscope 24 h after incubation. Photos of invade cells were taken (left panel) and Quantitation of invade cells was shown on right panel. e Increased Migration potential in cisplatin-resistant NSCLC cells. Cells indicated were seeded on 6-well plates and wound was made by scratch with 10uL tip. Closure distance was measured at 0 and 12 h after scratching. Quantitation analysis was shown on right panel. *p < 0.05, **p < 0.01, ***p < 0.001, compared to parental cells