Fig. 1

Spautin-1 suppresses the proliferation of PCa independent of USP10 and USP13. a Cell viability assay was performed in LNCaP, 22Rv1, C4–2, PC3 and DU145 PCa cells post various concentrations of Spautin-1 treatment for indicated hours. b Colony formation assay was performed in PCa cells post Spautin-1 (10 μM) treatment for two weeks. DM, DMSO. c Cell viability assay was performed in LNCaP, 22Rv1 and PC3 treated with control siRNA, USP10 siRNA, or USP13 siRNA for 72 h. d Western blot analysis was used to detect the expression of SKP2 and p27 in LNCaP and PC3 cells treated with Spautin-1 for 24 h. GAPDH was used as a loading control. e Cell viability assay was performed in LNCaP, 22Rv1 and PC3 treated with SKP2-C25 for 48 h. f Cell viability assay was performed in PC3 cells pre-transfected with control vector or SKP2 plasmid for 24 h before Spautin-1 (20 μM) treatment for 48 h. g Edu staining assay was performed to detect the proliferation ability of PC3 cells treated with Spautin-1, SKP2-C25, or 3-MA treatment for 24 h, or 48 h after subject to USP10 knockdown (KD) and USP13 KD. The staining of Edu in the nucleus indicates the cell in proliferation