Fig. 3

High dose of Spautin-1 triggers caspase-dependent apoptosis in PCa cells. a Flow cytometry analysis following Annexin V-FITC and PI staining was performed on PCa cells exposed to Spautin-1 for 24 h, or b subject to USP10 KD and USP13 KD for 48 h. Data of three independent repeats are summarized. ^*P<0.05 versus control treatment. c Western blot analysis was performed to detect the expression of PARP, Bim, Bax, Bcl-2 and C-Cas3 (cleaved caspase3) in PCa cells exposed to various doses of Spautin-1 (0, 5, 10, 20 μM) for 24 h (left), or Spautin-1 (20 μM) at various lengths of time (Right). d Cell viability analysis was performed on PCa cells exposed to Spautin-1 (20 μM) in the presence or absence of Z-VAD-FMK (50 μM). ^#P<0.05. e Apoptosis assay was performed on PC3 cells exposed to Spautin-1 (20 μM) in the presence or absence of Z-VAD-FMK (50 μM). f Shown are pooled data from three independent experiments. ^*P<0.05 versus control treatment; ^#P<0.05 versus Spautin-1 treatment. g Western blot analysis was performed to detect the expression of PARP and C-Cas3 in PC3 cells subject to Spautin-1 (20 μM) treatment in the presence or absence of Z-VAD-FMK (50 μM)