Fig. 4

JNK and ERK mediate Spautin-1-induced growth inhibition. a Western blot analysis was performed to detect the expression of phosphor-JNK, JNK, phosphorylated ERK (P-ERK), ERK, P-P38 and P38 in PCa cells exposed to various doses of Spautin-1 (0, 5, 10, 20 μM) for 24 h (left), or Spautin-1 (20 μM) at various time points (Right). b Immunofluorescence microscopy was performed to detect the expression and subcellular location of phospho-JNK and d phospho-ERK in 22Rv1 and PC3 cells exposed to Spautin-1 for 6 h. Scale bars represent 50 μm. c Cell viability assay was performed on PCa cells exposed to Spautin-1 (20 μM) in the presence or absence of SP600125 or SB230580 for 24 h. e Cell viability assay was performed on PCa cells exposed to Spautin-1 (20 μM) in the presence or absence of LY3214996 for 24 h