Fig. 7

a RNA levels of SNHG15 and control RNAs in nuclear and cytoplasmic fractions measured by qRT-PCR. The statistical analysis is performed by two-tailed Student’s t-test b Top: Silver stained gel with the proteins retained in the RNA pull-down experiment by SNHG15 and linc-p21 as negative control (CN), the differential band analyzed by mass spectrometry is indicated by an arrowhead; bottom: detection of the AIF protein by western blot in a replicate of the RNA pull-down experiment. c RNA immunoprecipitation using AIF monoclonal antibody and followed by qRT-PCR using three different primer pairs for SNHG15, and MALAT-1, U6, GAPDH and HPRT as negative controls. d AIF localization after SNHG15 inhibition using immunofluorescence antibody. Nuclei were stained with DAPI. e ROS levels of in LoVo cells transfected with the indicate siRNAs (p value< 0.05). f Cell viability determined by MTS of CRC cells (LoVo and HCT 116) after depletion or overexpression of SNHG15 treated with 8 μg/mL for 48 h (p value< 0.001). g Connection between AIF and the genes validated with most important pathway recognized. The statistical analysis is performed by two-tailed Student’s t-test and graphs shows mean ± SEM of values