Fig. 5

Overexpression of miR-199b simultaneously suppresses the activation of Akt and ERK signaling pathways both in vitro and in vivo. a Overexpression of miR-199b inhibited Akt and ERK signaling and stimulated apoptosis in A549 and H2122 NSCLC cell lines with K-Ras mutations. The indicated cells were transfected with negative oligonucleotide controls or miR-199b mimics. After 72 h of transfection, cells were subjected to Western blot analysis. b Aerosol delivery of miR-199b inhibited the Akt and ERK signaling pathways and stimulated apoptosis in lung tumors of K-Ras^LA1 transgenic mice. The indicated proteins were detected using immunohistochemistry, and the apoptotic cells were detected using TUNEL assay. c Inhibition of miR-199b activated Akt and ERK signaling. The indicated cells were transfected with negative oligonucleotide controls (NC) or antisense nucleotides of miR-199b. After 72 h of transfection, cells were subjected to Western blot analysis