Fig. 5

Fn14 enhances ubiquitylation and degradation of p53-R248Q by down-regulation of Hsp90. (a) qRT-PCR detecting the mRNA expression of p53-R248Q in HGSOC cells after infection with Fn14 lentivirus (b) and (c) Upon CHX treatment, western blot analysis detecting the expression of p53-R248Q in HGSOC cells after infection with Fn14 lentivirus. (d) and (e) Co-IP analysis detecting the level of ubiquitination p53 R248Q in HGSOC cells infected with Fn14 lentivirus. (f) and (h) western blot analysis detecting the expression of Hsp90 in HGSOC cells after infection with Fn14 lentivirus. (g) and (i) Co-IP analysis detecting the expression of mutp53-Mdm2-Hsp90 complex in HGSOC cells infected with Fn14 lentivirus. Unpaired Student’s t test was performed for comparisons between two groups. (j) Western blot analysis detecting the expression of p53-R248Q in HGSOC cells after transfection with Mdm2 siRNA