Fig. 1

Celastrol (Cel) inhibited cell viability in glioma cells. a Three glioma cell lines (U251, U87-MG and C6) were treated with celastrol (0, 0.3, 1, 3 and 10 μM) for 12, 24, 36 and 48 h. Cell viability was measured by CCK8 assay. b U251, U87-MG and C6 cells were treated with celastrol (0, 0.03, 0.1, 0.3 and 1 μM) for approximately 10 days. Cell colony formation was evaluated by a clone-formation assay. c Comparison of the effect of celastrol on three glioma cell samples with that on the astrocytes for 24 and 48 h. Three glioma cell lines and astrocyte cells were treated with celastrol (0, 0.3, 1, 3 and 10 μM) for 24 and 48 h. Cell viability was measured by CCK8 assay. Data are presented as the Mean ± SD (n = 3). **P < 0.01, ***P < 0.001, significantly different compared with the untreated control group