Fig. 4

Restored miR-26a enhances HBMEC proliferation, migration, tube formation, and angiogenesis. a proliferation of HBMECs transfected with miR-26a agomir or miR-26a antagomir detected using EdU assay (× 400); b migration of HBMEC transfected with miR-26a agomir or miR-26a antagomir assessed using transwell assay (× 400); c tube formation of HBMECs transfected with miR-26a agomir or miR-26a antagomir (× 400); d protein levels of VEGF, MMP-2 and MMP-9 of HBMEC transfected with miR-26a agomir or miR-26a antagomir measured using Western blot analysis; *, p < 0.05, compared with the agomir-NC group; #, p < 0.05, compared with the antagomir-NC group; all data were expressed as mean ± standard deviation; comparisons between two groups were analyzed using unpaired t-test; comparisons among multiple groups were analyzed by the one-way ANOVA; the experiment was repeated three times; ANOVA, analysis of variance; miR-26a, microRNA-26a; HBMECs, human brain microvascular endothelial cells; EdU, 5-ethynyl-2′-deoxyuridine; VEGF, vascular endothelial growth factor; MMP-2, matrix metalloproteinase-2; MMP-9, matrix metalloproteinase-9; NC, negative control