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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Glucocorticoid modulatory element-binding protein 1 (GMEB1) interacts with the de-ubiquitinase USP40 to stabilize CFLARL and inhibit apoptosis in human non-small cell lung cancer cells

Fig. 5

GMEB1 promoted the interaction between USP40 and CFLARL. a, b GMEB1 siRNA or plasmid were co-transfected with FLAG-CFLARL and HIS-USP40 in HEK293FT cells. Cells were harvested after 24 h for co-IP assay analysis. c HA-GMEB1–325-573 plasmid were co-transfected with FLAG-CFLARL and HIS-USP40 in HEK293FT cells. Cells were harvested after 24 h for co-IP assay analysis. d H460 and A549 cells were seeded in 6-well plates. GMEB1 plasmid and USP40 siRNA were co-transfected for 24 h; non-sense siRNA and pcDNA3.1 were transfected as control. Cells were harvested for western blot analysis. e A549 cells were seeded in 6-well plates. USP40 plasmid and GMEB1 siRNA were co-transfected for 24 h; non-sense siRNA and pcDNA3.1 were transfected as control. Cells were harvested for western blot analysis

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