Fig. 4

miRNA profiling of CD166⁺ OSCs. a Venn analyses of the up- and downregulated miRNAs in CD166⁺ OSCs from primary OS tissues (left, yellow shaded; n = 3) and in CD166⁻ cells with TGFβ1 (right, blue shaded), compared with CD166⁻ cells, are shown. miRNAs that were significantly and commonly deregulated in both CD166⁺ OSCs and CD166⁻ cells with TGFβ1 are shown in the overlapping area (green shaded). Only those miRNAs whose expression levels displayed greater than 2-fold decreases or increases were further studied. The table shows a summary of the significantly differentially expressed miRNAs in the overlapping area with fold change. b Relative expression of miR-499a in CD166⁺ OSCs, CD166⁻ cells with or without TGFβ1 from OS-1 and OS-5 were examined by qPCR. Note: Columns, mean of three individual experiments; SD,**, P < 0.01