Fig. 2

Conditioned medium of senescent thyrocytes and thyroid tumor cell lines triggers M2-like macrophage polarization. In vitro hrM-CSF-generated control macrophages (a), human purified monocytes treated with the conditioned medium of proliferating (PTh) and senescent (STh) thyrocytes, collected at the indicated time points after 4OHT treatment (b), and normal Nthy-ori 3–1 (Nthy) and tumor (TuC) thyroid cell lines (c) were analyzed by FACS for the expression of MHCII and CD206 (top panels) and by ELISA for the secretion of CCL17 (bottom panels). Histograms represent mean + standard deviation of 3–4 independent experiments. Statistical significance was determined by unpaired t test *p < 0.05, ** p < 0.01, ***p < 0.001. MFI: mean fluorescence intensity