Fig. 7

PTGS2 and M2 markers expression in human thyroid tissues. a Boxplots showing PTGS2 and CD163 gene expression in normal thyroids and tumor samples derived from GEO datasets (GSE3467, GSE6004, GSE33630 and GSE76039). Statistical significance by Mann Whitney test; * P < 0.05, ** P < 0.005, *** P < 0.0001. b Correlation by Spearman coefficient between PTGS2 and CD163 expression in the same series. c Heatmap showing PTGS2 and M2 markers (CD163 and CD206) expression across the same series; mRNA levels were expressed as normalized data (row Z-score). Class was established based on PTGS2 and CD163 median expression (high ≥ median; low < median); class coexpression high: coordinated PTGS2 and CD163 high; class coexpression low: coordinated PTGS2 and CD163 low; class PTGS2-only: PTGS2high and CD163 low; and class M2-only: CD163 high and PTGS2 low. d Barplot showing histotype distribution across PTGS2/CD163 expression classes. e Heatmap showing PTGS2 and M2 markers (CD163 and CD206) expression in GSE76039 dataset; PTGS2/CD163 expression class, histotype and M2 score (indicative of macrophages infiltration) is indicate for each sample. f Correlation between PTGS2 expression an M2 score in GSE76039 dataset