Fig. 2

FOXM1 regulates KIF4A expression. a, b HEK293T cells were transfected with overexpression plasmids for specific isoforms of FOXM1 for 48 h and then harvested for western blotting with indicated antibodies (a) and quantitative real-time PCR (b). c, d HepG2, Huh7, and Sk-Hep1 cells were transfected with pcDNA 3.1-Myc/HisA-vector or pcDNA 3.1-Myc/HisA-FOXM1c plasmids for 48 h and then harvested for western blotting with indicated antibodies (c) and quantitative real-time PCR (d). e HEK293T cells transfected with shRNA-Control or shRNA-FOXM1 constructs for 48 h were harvested for western blotting with indicated antibodies. f, g Huh7, and Hep3B cells were transfected with sh-Control or sh-FOXM1 for 48 h and then harvested for western blotting (f) with indicated antibodies and quantitative real-time PCR (g). CENPF and CCNB1 were used as a positive control. Data are shown as mean ± SD (n = 3). *p < 0.05, **p < 0.01