Fig. 3

FOXM1 directly binds to the KIF4A promoter and activates gene transcription. a Map of FOXM1 binding site sequence. b Schematic illustration of four potential FOXM1 binding sites (BS1, BS2, BS3, and BS4) in the KIF4A promoter (top). The 9-base pair sequence of the FOXM1 consensus site and sequences of four FOXM1-binding sites in the KIF4A promoter are shown. Chromatin was isolated from HepG2 cells, and ChIP assay was performed with goat IgG control and FOXM1-specific antibodies (bottom). c Full-length KIF4A luciferase construct was transfected into HepG2 cells with empty vector or the FOXM1 overexpression plasmid for 48 h. Transcriptional activation was analyzed with the dual luciferase reporter assay, with human CCNB1-luciferase construct used as a positive control. d Luciferase constructs for wild-type, or mutant KIF4A promoter was transfected into HepG2 cells along with FOXM1 overexpression plasmid or control vector for 48 h. Transcriptional activation was analyzed with the dual luciferase reporter assay. Results were normalized to Renilla luciferase activity and are expressed as fold change in luciferase activity relative to the control. Data represent the mean ± SD of three independent experiments. **p < 0.01