Fig. 5

KIF4A is required for FOXM1-induced HCC cell proliferation in vitro. a, b HepG2 cells were infected with FOXM1 overexpression (FOXM1) and KIF4A knockdown (sh-KIF4A) lentiviral vectors and cells were harvested for quantitative real-time PCR (a) or western blotting (b). c–f Proliferation of HepG2 cells stably expressing FOXM1 + sh-KIF4A was evaluated with the EdU (c), colony formation (d), cell growth (e), and CCK-8 (f) assays. g–i HepG2 cells stably expressing FOXM1 + sh-KIF4A were subjected to cell cycle analysis (g); the fraction of cells in each phase (h) and number of multinucleate cells (i) were shown as a histogram. Data represent mean and SD of three independent experiments. *p < 0.05; **p < 0.01