Fig. 2

Bmi1 inhibits cell surface expression of MICA/B and blocks NK cell-mediated lysis in pancreatic cancer cells. a-b Expression of Bmi1 at mRNA and protein levels were detected by q-PCR (a) and Western blot (b) in PANC-1 and SW1990 cell lines after transfection of Bmi1 cDNA. c-d Expression of MICA/B at mRNA and protein levels were detected by q-PCR (c) and Western blot (d) after Bmi1 overexpression. e MFI (folds) of MICA/B detected by flow ctyometry was evaluated with a Student t test from three independent experiments. f Representative histograms of flow cytometry demonstrating MICA/B expression in pancreatic cell after Bmi1 overexpression. g Immunohistochemistry results reveal that cell surface MICA/B show low expression where Bmi1 is high expressed in pancreatic cancer tissues. h The killing effect of NK cells on pancreatic cancer cells overexpression Bmi1. The graphs show representative results from three independently repeated experiments. The data were from three independently repeated experiments. **P < 0.01; *P < 0.05