Fig. 2

WZ35 and cisplatin cooperated to trigger ROS-dependent apoptosis in gastric cancer cells. (a-c) SGC-7901 or BGC-823 cells were treated with WZ35 or cisplatin alone or their combination at the indicated doses. At 2 h after treatment, intracellular ROS generation was determined by flow cytometry. (d-f) SGC-7901 or BGC-823 cells were pretreated with 5 mM NAC for 2 h before the combined treatment. Intracellular ROS generation was measured by flow cytometry. (g) SGC-7901 or BGC-823 cells were pretreated with 5 mM NAC for 2 h before exposure to WZ35 and cisplatin combination. At 24 h after treatment, the cell viability was determined by MTT assay. (h) SGC-7901 or BGC-823 cells were pretreated with 5 mM NAC for 2 h before exposure to WZ35 and cisplatin combination for 24 h. Percentage of cell apoptosis was determined by flow cytometry. The percentage of apoptotic cells in the treatment groups was calculated. Data represent similar results from three independent experiments. (* p < 0.05, ** p < 0.01)