BTF3 overexpression predicts poor outcome of PCa (a) Representative IHC images of BTF3 expression of benign prostate tissue and PCa tissue with different Gleason score in Qilu cohort. GS = Gleason score. (b-e) The percentage of BTF3 expression distributed in PCa cases with different Gleason score (g), stage (h), metastasis(i) and clinical recurrence(i). REC = recurrence. (f) Representative HE and IHC images of NEPC tissue showing SYN and BTF3 expression. (g) Oncoprint of amplification and mRNA upregulation of BTF3 gene in Beltran Prostate cohort according to the cBioPortal database. (h) Kaplan–Meier survival analysis of PCa cases from Qilu cohort stratified by high and low BTF3 expression levels (p = 0.006, Log-rank test). (i) Kaplan–Meier survival analysis of PCa cases from GEPIA cohort according to high and low BTF3 expression levels (p = 0.03, Log-rank test). (j) Nomograms based on Gleason score, stage, Pre PSA (4–10–20-30-40-50-60-70-80-90-100 ng/ml), BTF3 expression and Age for predicting survival probability of PCa patients.